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    Please use this identifier to cite or link to this item: http://ir.lib.ksu.edu.tw/handle/987654321/3315

    Title: Investigating the dynamics of organelle-actin assembly with dielectrophoresis
    Authors: 陳恕行(ChenShu-Hsing)
    Keywords: dielectrophoresis
    repositioning of chloroplasts
    chloroplast-actin binding
    Date: 2009-03-11
    Issue Date: 2009-08-14 00:14:26 (UTC+8)
    Abstract: Dielectrophoresis is a technique of using high-frequency electric fields to manipulate dielectricparticles. This work applies this technique to study the changes of intracellular properties in responseto environmental changes. In a number of plant cells, chloroplasts change their positions foroptimizing photosynthetic light absorption. Only between 2 and 20% of the light energy absorbed byalgae and higher plants is actually used in CO2 fixation. Comparing with other micro-manipulativemethods, dielectrophoresis is a relative simple method for investigating the microscopicmechanisms involved in chloroplast repositioning.Leaves of Egeria densa were subjected to different lighting conditions before conductingdielectrophoresis. Under dim light, chloroplasts situate on the periclinal wall. Under high-intensitylight, chloroplasts move from the periclinal wall to the anticlinal walls. Under electric fields,chloroplasts treated with different lighting condition exhibit different motions. It is expected thatactin configuration as well as chloroplast-actin binding strength contributes to the repositioning ofchloroplasts.
    Relation: 2007, ICAM
    Appears in Collections:[機械工程系所] 會議論文

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